Study on the molecular epidemiology of SHV type beta-lactamase-encoding genes of multiple-drug-resistant acinetobacter baumannii / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 425-427, 2004.
Article
en Zh
| WPRIM
| ID: wpr-342293
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyse the plasmid-mediated SHV type beta-lactamases-encoding genes sequence and to identify its subtype of multiple-drug-resistant acinetobacter baumannii isolated from Huzhou district, Zhejiang province, China.</p><p><b>METHODS</b>Sixty strains of acinetobacter baumannii were isolated from hospitalized patients between Jul, 2000 and Dec, 2002. Susceptibility of antimicrobial agents and confirmatory tests for Extended-spectrum beta-lactamases (ESBLs) were tested by microdilute method. SHV type beta-lactamases-encoding genes were tested by polymerase chain reaction (PCR). SHV sequences of acinetobacter baumannii HZ02 and HZ10 strains were detected by ABI automated sequencer and were analysed to compare with SHV genes that had been published in GenBank.</p><p><b>RESULTS</b>Eighteen (30.0%) strains of acinetobacter baumannii isolated between Jun, 2001 and Jan, 2002 were carrying SHV beta-lactamases resistant gene of plasmids. Detected SHV sequences of acinetobacter baumannii HZ02 strain and HZ10 strain had 825 and 833 nucleotides respectively and had the same gene sequence as the gene encoding SHV-12 subtype of ESBLs discovered in Switzerland.</p><p><b>CONCLUSIONS</b>Thirty percentage of the clinically isolated acinetobacter baumannii were carrying SHV type (extended-spectrum) beta-lactamases resistant gene of plasmids and causing an outbreak in hospital and was discovered to have carried the strains of SHV-12 subtype producing ESBLs gene in acinetobacter baumannii which was the first reported case in the world.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Beta-Lactamasas
/
Infecciones por Acinetobacter
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ADN Bacteriano
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Datos de Secuencia Molecular
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Secuencia de Bases
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China
/
Epidemiología
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Secuencia de Aminoácidos
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Clasificación
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Análisis de Secuencia de ADN
Tipo de estudio:
Screening_studies
Límite:
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Epidemiology
Año:
2004
Tipo del documento:
Article