Determination of five triterpenic acids from Salvia chinensis of different parts by RP-HPLC-PDA / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 4379-4382, 2014.
Article
en Zh
| WPRIM
| ID: wpr-341850
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a RP-HPLC-PDA method for determination of five triterpenic acids (pomolic acid, hawthorn acid, corosolic acid, oleanolic acid and ursolic acid) in different parts of Salvia chinensis.</p><p><b>METHOD</b>The isocratic elution and separation was achieved on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm), using acetonitrile-water (90:10) as the mobile phase at a flow rate of 0.8 mL x min(-1). The detection wavelength and column temperature were set at 205 nm and 28 degrees C, respectively.</p><p><b>RESULT</b>The calibration curves of pomolic acid, hawthorn acid, corosolic acid, oleanolic acid and ursolic acid were linear over the ranges of 0.096 0-2.400, 0.1230-3.075, 0.2420-6.050, 0.2830-7.075 and 0.2730-6.825 microg (r = 0.9998, 0.9997, 0.9999, 0.9995, 0.9999), respectively. The average recoveries were 98.43%, 98.13%, 100.6%, 98.19% and 99.15%%, respectively, with RSD (n=6) being 1.3%, 0.67%, 1.2%, 0.87% and 0.43%.</p><p><b>CONCLUSION</b>The proposed method is so simple and highly reproducible that it promises to be applicable for determination of major triterpenic acids in S. chinensis.</p>
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Base de datos:
WPRIM
Asunto principal:
Plantas Medicinales
/
Triterpenos
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Medicamentos Herbarios Chinos
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Química
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Cromatografía Líquida de Alta Presión
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Salvia
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Métodos
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2014
Tipo del documento:
Article