Changes in Ca2+-ATPase in a guinea pig endolymphatic hydrops model / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1191-1195, 2002.
Article
en En
| WPRIM
| ID: wpr-340359
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the localization of Ca(2+)-ATPase (Ca(2+) pump) in the cochlear and its change after endolymphatic hydrops, and to study the relationship between compound action potential (CAP) threshold and the Ca(2+)-ATPase activety.</p><p><b>METHODS</b>The left endolymphatic sac was ablated to induce endolymphatic hydrops in fourteen healthy guinea pigs with normal action potential thresholds measured after a sliver ball electrode placed on the round window. Ca(2+)-ATPase activity was studied cytochemically using a lead citrate reaction in control and hydropic ears. The reaction product was lead phosphate particles as an expression of Ca(2+)-ATPase activity, observed with an eletron microscope.</p><p><b>RESULTS</b>Ca(2+)-ATPase activity is mainly found on the endolymphatic surface of Reis sner's membrane, the stereocilia and cuticular plate of inner and outer hair cells, and along the infolded plasma membrane of strial marginal cells. CAP thresholds of filtered click are increased and Ca(2+)-ATPase activity significantly decreased after endolymphatic hydrops in the mentioned locations.</p><p><b>CONCLUSIONS</b>CAP thresholds are increased and Ca(2+)-ATPase activity are significantly decreased in the cochlea after endolymphatic hydrops. These results suggest that there is a negative correlation between them.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Sodio
/
Potenciales de Acción
/
Calcio
/
Cóclea
/
ATPasas Transportadoras de Calcio
/
Hidropesía Endolinfática
/
Cobayas
/
Metabolismo
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Chinese Medical Journal
Año:
2002
Tipo del documento:
Article