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Characterization of S gene of a strain of hantavirus isolated from Apodemus peninsulae in Heilongjiang Province / 病毒学报
Chinese Journal of Virology ; (6): 517-521, 2012.
Article en Zh | WPRIM | ID: wpr-340013
Biblioteca responsable: WPRO
ABSTRACT
In order to study the molecular characterization of the hantavirus isolated from Apodemus peninsulae in Heilongjiang Province, the S gene of a new strain NA33 was amplified, sequenced and analyzed. The results showed that the complete nucleotide sequence of the S gene of NA33 strain was composed of 1 693 nucleotides with TA-rich. The S gene contained one ORF, starting at position 37 and ending at position 1 326, encoding the N protein of 429 amino acid residues, and in line with HTN-based coding. Sequence comparison of the S genes between NA33 and reference hantavirus strains showed that NA33 was more homologous to Amur-like viruses than to the Hantaan (HTN) viruses or the other hantaviruses. Phylogenetic analysis of the amino acid sequence of N proteins showed that NA33 was clustered into the group of Amur-like viruses and was more similar to Far East Russia and Jilin strains isolated from Apodemus peninsulae. The phylogenetic tree indicated a certain degree of host-dependent characteristics and geographical aggregation characteristics of hantanviruses. Furthermore, the amino acid sequence of N protein of NA33 had the conserved amino acid sites of Amur-like viruses. In conclusion, Apodemus peninsulae carried Amur-like viruses in Heilongjiang province and was an important infectious source of hemorrhagic fever with renal syndrome (HFRS).
Asunto(s)
Texto completo: 1 Base de datos: WPRIM Asunto principal: Filogenia / Enfermedades de los Roedores / Virología / Datos de Secuencia Molecular / Química / China / Proteínas del Envoltorio Viral / Clasificación / Orthohantavirus / Homología de Secuencia de Aminoácido Límite: Animals / Humans País/Región como asunto: Asia Idioma: Zh Revista: Chinese Journal of Virology Año: 2012 Tipo del documento: Article
Texto completo: 1 Base de datos: WPRIM Asunto principal: Filogenia / Enfermedades de los Roedores / Virología / Datos de Secuencia Molecular / Química / China / Proteínas del Envoltorio Viral / Clasificación / Orthohantavirus / Homología de Secuencia de Aminoácido Límite: Animals / Humans País/Región como asunto: Asia Idioma: Zh Revista: Chinese Journal of Virology Año: 2012 Tipo del documento: Article