Comparison of different molecular assays for the rapid detection of enterovirus 71 (EV71) / 病毒学报
Chinese Journal of Virology
; (6): 670-674, 2012.
Article
en Zh
| WPRIM
| ID: wpr-339987
Biblioteca responsable:
WPRO
ABSTRACT
Molecular detection of enterovirus (EV)71 RNA based on PCR methods is a quick and sensitive approach. At present, different PCR-based methods for EV71 RNA detection are available, but comparisons of results obtained using different approaches are limited. This study is to compare the analytical sensitivity and specificity of different real-time reverse transcription-polymerase chain reaction (rRT-PCR) and conventional reverse transcription-polymerase chain reaction (cRT-PCR) assays for enterovirus and EV71 detection, Altogether, three rRT-PCR assays and one cRT-PCR assay targeting the 5'UTR gene for universal detection of enterovirus; two rRT-PCR assays andone cRT-PCR assay targeting the VP1 gene for specific detection of EV 71 were examined. All assays showed good specificity. The detection sensitivity ranged from 8.19 x 10 to 8.19 x 10(5) copy equivalents. In general, rRT-PCR assays were more sensitive than cRT-PCR assays. All rRT-PCR assays showed 100% sensitivity for clinical specimens.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Virología
/
Sensibilidad y Especificidad
/
Clasificación
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Enterovirus Humano A
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Diagnóstico
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Infecciones por Enterovirus
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Reacción en Cadena en Tiempo Real de la Polimerasa
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Genética
/
Métodos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2012
Tipo del documento:
Article