Effect of Shenfu injection on nuclear factor-kappaB during myocardial ischemia/reperfusion injury in rats / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
; (6): 200-204, 2005.
Article
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| WPRIM
| ID: wpr-338613
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effects of Shenfu injection on the concentrations of plasma tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), activity of Nuclear Factor kappa B (NF-kappaB) and heart tissue ultrastructure during myocardial ischemia/reperfusion (I/R) injury in rats and its potential mechanism.</p><p><b>METHODS</b>Myocardial ischemia/reperfusion (I/R) was produced by ligation and release of the left anterior descending coronary artery. Ischemia lasted for 30 min and reperfusion for 60 min. Twenty-four healthy male SD rats weighing 230-280 g were randomly divided into three groups (n = 8, each): Group I (Sham-operation group); Group II (I/R group); Group III (Shenfu group), in which Shenfu injection (10 ml/kg) was intraperitoneally injected 30 min before ischemia in animals with I/R. The plasma concentrations of IL-6 and TNF-alpha were measured by ELISA, and the heart was harvested for determination of NF-kappaB levels by Ecl-western blot analysis. Electron microscopy was used to study its ultrastructure.</p><p><b>RESULTS</b>After reperfusion, NF-kappaB binding activity in myocardial nuclei and the plasma concentrations of IL-6 and TNF-alpha were significantly increased in Group II, compared with Group I (P < 0.01), and they were markedly reduced in Group III, compared with Group II (P < 0.01). In addition, electron microscopic examination showed more serious injury of the myocardium ultrastructure in Group II, while in Group III the myocardial ultrastructure was similar to normal state.</p><p><b>CONCLUSIONS</b>Shenfu injection inhibits NF-kappaB activity in I/R myocardium and leads to down-regulation of proinflammatory cytokine expression, which might be one of the molecular mechanisms of Shenfu injection in cardioprotection.</p>
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Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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Sangre
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Medicamentos Herbarios Chinos
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Daño por Reperfusión Miocárdica
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FN-kappa B
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Interleucina-6
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Factor de Necrosis Tumoral alfa
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Ratas Sprague-Dawley
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Quimioterapia
Límite:
Animals
Idioma:
En
Revista:
Chinese Journal of Traumatology
Año:
2005
Tipo del documento:
Article