Relationship between germ cells apoptosis and alterations of total antioxidant capacity, nitric oxide level and nitric oxide synthase activity in testes of rats submitted to alcohol drinking / 中华男科学杂志
National Journal of Andrology
; (12): 21-24, 2006.
Article
en Zh
| WPRIM
| ID: wpr-338377
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between germ cells apoptosis and alterations of total antioxidant capacity (T-AOC), nitric oxide(NO) level and nitric oxide synthase (NOS) activity in the testes of rats submitted to alcohol drinking.</p><p><b>METHODS</b>Twenty healthy male Sprague-Dawley rats (3 months old) were randomly divided into two groups: control group and experimental group. 50% alcohol and distilled water were administered intragastrically at a dose of 10 ml/kg body weight to two groups of rats respectively. After twenty-six days, the biochemical parameters (T-AOC, NO level and NOS activity) were measured with spectrophotometric determination. The TdT-mediated dUTP-X nick end labeling (TUNEL) technique was used to detect germ cells apoptotic index (AI).</p><p><b>RESULTS</b>Compared with the control group, AI was significantly higher (P < 0.01) in the experimental group; T-AOC level reduced obviously (P < 0.01), but NO level and NOS activity increased predominantly ( P < 0.01).</p><p><b>CONCLUSION</b>The excessive production of NO caused by the increasing of NOS activity and the decreasing of T-AOC may be the main causes that alcohol overtaking induces germ cells apoptosis.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Testículo
/
Distribución Aleatoria
/
Ratas Sprague-Dawley
/
Apoptosis
/
Óxido Nítrico Sintasa
/
Biología Celular
/
Etanol
/
Toxicidad
/
Células Germinativas
/
Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
National Journal of Andrology
Año:
2006
Tipo del documento:
Article