Study on the relationship between heat shock protein 70 and toll-like receptor-4 of monocytes / 华中科技大学学报（医学）（英德文版）
J. huazhong univ. sci. tech. med. sci
; (6): 560-562, 2004.
Article
en En
| WPRIM
| ID: wpr-336979
Biblioteca responsable:
WPRO
ABSTRACT
To explore the relation between human heat shock protein 70 (hsp70) and TLR4 in human monocytes in vitro, human monocytes were stimulated with various concentrations of HSP70, and TNF-alpha production in supernatants was measured by ELISA. Pre-incubated with or without anti-TLR4 mAb, and stimulated with hsp70 (5.0 microg/ml), NF-kappaB p65 of human monocytes in different time points were detected by immunohistochemistry and monocyte surface expression of TLR4 was measured by flow cytometry. After the human monocytes were pre-incubated with various concentrations of anti-TLR4 and stimulated with hsp70 (5.0 microg/ml), TNF-alpha production in supernatants was measured. The results showed that hsp70 enhanced NF-kappaB activation, which was clearly inhibited by anti-TLR4, with the positive cell ratios being 67.44%, 39.17%, 31.56% and 28.05 %, respectively. TLR4 was rapidly down-regulated in the presence of hsp70. MFI of TLR4 on monocytes in different time points were 87.77 +/- 5.38, 78.16 +/- 6.01 and 45.17 +/- 4.97 (P<0.05), 26.98 +/- 5.83 (P<0.01), respectively. Moreover, hsp70-induced TNF-alpha production by human monocytes was inhibited by anti-TLR4. It is suggested that TLR4 is involved in the hsp70-mediated activation of innate immunity.
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Base de datos:
WPRIM
Asunto principal:
Técnicas In Vitro
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Monocitos
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Factor de Necrosis Tumoral alfa
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Proteínas HSP70 de Choque Térmico
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Factor de Transcripción ReIA
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Receptor Toll-Like 4
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Metabolismo
Límite:
Humans
Idioma:
En
Revista:
J. huazhong univ. sci. tech. med. sci
Año:
2004
Tipo del documento:
Article