A method for simultaneous assay of propulsion and absorption in small intestine / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 430-449, 2012.
Article
en Zh
| WPRIM
| ID: wpr-336773
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a method for simultaneous assay of propulsion and absorption in small intestine.</p><p><b>METHODS</b>The mice were administrated through gastric tube with mixed reagents containing 0.12% phenol red, D-xylose (1.25%, 2.5% and 5%) and 15% gelatin. The influence of phenol red on D-xylose absorption and the influence of D-xylose on small intestine propulsion rate were investigated by measuring serum concentration of D-xylose with phloroglucinol method.</p><p><b>RESULTS</b>At 10 min, no significant difference was found between 5% D-xylose mixed reagent group and 5% D-xylose control. At 15 min, small intestine propulsion rate in 5% D-xylose mixed reagent group, but not in 2.5% and 1.25% D-xylose mixed reagent groups, was significantly higher than in phenol red control (P<0.05).</p><p><b>CONCLUSION</b>Gastric administration of mixed reagent containing 0.12% phenol red, 5% D-xylose and 15% gelatin can simultaneously assay propulsion and absorption of small intestine in mice.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Peristaltismo
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Fenolsulfonftaleína
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Fisiología
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Xilosa
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Bioensayo
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Farmacocinética
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Absorción Intestinal
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Intestino Delgado
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Metabolismo
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Métodos
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2012
Tipo del documento:
Article