Construction and immunogenicity study of DNA vaccine expressing human H5N1 influenza virus hemagglutinin / 病毒学报
Chinese Journal of Virology
; (6): 366-370, 2007.
Article
en Zh
| WPRIM
| ID: wpr-334881
Biblioteca responsable:
WPRO
ABSTRACT
Based on the first isolated human H5N1 influenza virus strain A/Anhui/1/2005 in China, HA and HA1 genes were amplified and cloned into the eukaryotic expression vector pStar. The recombinant plasmids pStar-HA and pStar-HA1 were transfected into COS7 cells. Western blot and IFA showed that the two recombinant DNA plasmids were successfully expressed in eukaryotic cells. BALB/c mice were immunized with the plasmids DNA by intramuscular injection. Anti-HA specific antibody in peripheral blood of immunized mice was tested by ELISA. The results showed that the recombinant plasmids successfully induced the anti-HA humoral immune response, and there was no significant difference between HA and HA1 as immunogen. This work provides basis for future development of novel avian flu vaccine.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Sangre
/
Inmunoglobulina G
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Vacunas contra la Influenza
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Chlorocebus aethiops
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Células COS
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Vacunas de ADN
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Glicoproteínas Hemaglutininas del Virus de la Influenza
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Alergia e Inmunología
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Subtipo H5N1 del Virus de la Influenza A
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Genética
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2007
Tipo del documento:
Article