Genome cloning and sequence analysis of duck circovirus / 病毒学报
Chinese Journal of Virology
; (6): 138-143, 2008.
Article
en Zh
| WPRIM
| ID: wpr-334834
Biblioteca responsable:
WPRO
ABSTRACT
To reveal the molecular biological characteristics of genome of circovirus in infected ducks, two nucleotide fragments were amplified by overlapping PCRs using DNA extracted from various tissues of ducks. After they had been assembled together, the nucleotide components, the genome organization and the phylogenetic scale of the sequence were analyzed. The results showed that the obtained sequence is a circular DNA with a total length of 1995nt. It contains 6 open reading frames (ORFs), and shares a high identity of 97.4% with the MuDCV circovirus sequence presented in GenBank (AY228555). These results indicate that the amplified product stems from duck circovirus sequence.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Filogenia
/
Variación Genética
/
Virología
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Clasificación
/
Clonación Molecular
/
Análisis de Secuencia de ADN
/
Circovirus
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2008
Tipo del documento:
Article