Effect of inducible nitric oxide synthase on intestinal microcirculation in endotoxic shock / 生理学报
Sheng Li Xue Bao
; (6): 39-44, 2005.
Article
en En
| WPRIM
| ID: wpr-334208
Biblioteca responsable:
WPRO
ABSTRACT
To investigate the changes of intestinal microcirculation in endotoxic shock and the effect of inducible nitric oxide synthase (iNOS) on intestinal microcirculation, endotoxic shock was induced by intravenous injection of lipopolysaccharide (LPS) in mice. Mean arterial pressure (MAP) was monitored throughout the experimental procedure. The velocity and flux of red blood cell (RBC) in villus tip arteriole and capillaries were measured by FITC-labeled erythrocytes and intravital microscopy. The effect of iNOS was determined by targeted disruption of mice iNOS-gene and administration of S-methylthiourea sulfate (SMT), a selective inhibitor of iNOS, before LPS injection. No significant differences in MAP, RBC velocity and flux at baseline were found among wild type mice, SMT pretreated mice and iNOS-gene knockout mice. LPS induced a dramatic fall of MAP in wild type mice. The decrease of MAP was significantly restored in iNOS-gene knockout mice and in wild type mice received SMT before LPS injection. The velocity and flux of RBC in villus tip arteriole and capillaries decreased markedly after LPS injection in wild type mice, while significantly higher velocity and flux of RBC were found in iNOS-gene knockout mice and SMT-pretreated mice both 60 and 120 min after LPS injection. The results demonstrate that iNOS plays an essential role in the intestinal microcirculation disturbance which occurs in endotoxic shock.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Fisiología
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Choque Séptico
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Lipopolisacáridos
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Ratones Noqueados
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Óxido Nítrico Sintasa de Tipo II
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Genética
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Intestinos
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Microcirculación
Límite:
Animals
Idioma:
En
Revista:
Sheng Li Xue Bao
Año:
2005
Tipo del documento:
Article