Fluorescence resonance energy transfer analysis for detecting protein kinase C activation / 南方医科大学学报
Journal of Southern Medical University
; (12): 1867-1870, 2011.
Article
en Zh
| WPRIM
| ID: wpr-333794
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a sensitive and direct method for detecting the activation of protein kinase C (PKC) using fluorescence resonance energy transfer (FRET) technique.</p><p><b>METHODS</b>HEK293 cells were transfected with C kinase activity reporter (CKAR) plasmid or/and parathyroid receptor 1 plasmid , and after incubation for 72 h, the fluorescence resonance energy transfer was measured with or without parathyroid or TPA stimulation.</p><p><b>RESULTS</b>TPA reduced the efficiency of FRET and increased the emission ratio of CFP/YFP (C/Y) in HEK293 cells transfected with CKAR. PTH(1-34) could increase the emission ratio of C/Y in HEK293 cells co-transfected with CKAR and PTHR1 but not in cells transfected with CKAR.</p><p><b>CONCLUSION</b>FRET analysis using CKAR can be utilized to detect the activation of PKC, which provides a useful means for studying the signaling pathways associated with PKC.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Proteínas Bacterianas
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Proteína Quinasa C
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Química
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Genes Reporteros
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Transferencia Resonante de Energía de Fluorescencia
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Activación Enzimática
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Células HEK293
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Proteínas Luminiscentes
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Metabolismo
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Métodos
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Article