Diverse components of voltage-gated calcium channel currents in primary cultured hippocampal neurons / 生理学报
Sheng Li Xue Bao
; (6): 687-694, 2012.
Article
en Zh
| WPRIM
| ID: wpr-333153
Biblioteca responsable:
WPRO
ABSTRACT
The present study was to record and separate the voltage-gated calcium channel currents (VGCCs) in primary cultured hippocampal neurons. An improved method is described for efficient and stable recording of VGCCs from primary cultured hippocampal neurons. The procedure allows the obtained hippocampal neurons overcome the defects from acutely isolated hippocampal neurons and currents do not rundown apparently after 20 min of membrane rupture. Using whole-cell configurations of patch clamp technique, it is shown that the method does not damage the neuron membrane electrical properties, and successfully records a variety of VGCCs components in hippocampal neurons (such as L, N, P/Q, T, etc.).
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Fisiología
/
Canales de Calcio
/
Células Cultivadas
/
Calcio
/
Técnicas de Placa-Clamp
/
Biología Celular
/
Cultivo Primario de Células
/
Hipocampo
/
Neuronas
Límite:
Animals
Idioma:
Zh
Revista:
Sheng Li Xue Bao
Año:
2012
Tipo del documento:
Article