Biological activities of recombinant human interferon Epsilon / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 227-231, 2005.
Article
en Zh
| WPRIM
| ID: wpr-333035
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct a novel recombinant rhIFN-epsilon155ser, and study its biological activities.</p><p><b>METHODS</b>The whole sequence of rhIFN-epsilon was artificially synthesized and some codons were altered according to the preferred codon using of E.coli. The sequence was cloned into plasmid vector pBV220 to express in E.coli DH5alpha. After purification and re-folding of rhIFN-epsilon155ser inclusion body, the final product was tested for its biological activities, including anti-viral, anti-proliferative and NK cell enhancing activities. At the same time, by using DNA microarray biochips, the gene expression patterns in the rhIFN-epsilon155ser and rhIFN-alpha2b treated cells were compared and analyzed.</p><p><b>RESULTS</b>The re-built rhIFN-epsilon155ser sequence was expressed in E.coli as a form of inclusion body. After purified and re-folded, the rhIFN-epsilon155ser protein reached a purity of above 95%. The rhIFN-epsilon155ser protein had a specific anti-viral activity of about 6 x 10(5) IU/mg in WISH/VSV system. Its anti-proliferative activity and NK cell enhancing activities in vitro seemed to be lower than that of rhIFN-alpha2b. Data obtained from microarray biochips indicated that there were 283 pieces increasing 2 folds and 1489 pieces decreasing 2 folds among totally 22,278 pieces of human genes were found in the rhIFN-epsilon155ser treated cells; more changes in gene expression pattern were detected in the rhIFN-alpha treated cells.</p><p><b>CONCLUSION</b>A novel recombinant rhIFN-epsilon155ser was constructed, which belonged to type 1 interferon. The biological activities of rhIFN-epsilon155ser were compared with rhIFN-alpha2b. The changes of gene expression pattern in the interferon treated cells were detected, analyzed and discussed.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Antivirales
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Farmacología
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Plásmidos
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Proteínas Recombinantes
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Células HeLa
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Células Asesinas Naturales
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Pruebas de Sensibilidad Microbiana
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Expresión Génica
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Interferones
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Clonación Molecular
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2005
Tipo del documento:
Article