Screening of the genes of hepatitis B virus e antigen interacting proteins / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 271-274, 2005.
Article
en Zh
| WPRIM
| ID: wpr-333024
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen and clone the genes in hepatocytes which encode protein that can interact with hepatitis B e antigen(HBeAg) by yeast-two hybridization.</p><p><b>METHODS</b>Recombined HBeAg bait plasmid (pGBKT7-eAg) was transformed into yeast AH l09, followed by mating with yeast Yl87 containing liver cDNA library plasmid in 2 x YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-Ade-His) which contains X-a-gal for selecting positive blue clones. Then positive clones were selected and plasmids were prepared and sequenced. Finally, bioinformatics analysis was performed.</p><p><b>RESULTS</b>Totally 245 positive colonies were selected and 101 colonies were sequenced. Through sequences alignment, 6 novel genes and 35 recorded genes were screened.</p><p><b>CONCLUSION</b>Genes of HBeAg interacting proteins have been cloned successfully, which brings some new clues for further studies on the biological functions of HBeAg and the related proteins.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plásmidos
/
Unión Proteica
/
Transformación Genética
/
Biblioteca de Genes
/
Técnicas del Sistema de Dos Híbridos
/
Genética
/
Antígenos e de la Hepatitis B
/
Hígado
/
Metabolismo
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2005
Tipo del documento:
Article