Impact of transfusion of apoptotic and necrotic thymocytes on the survival of mice with sepsis / 南方医科大学学报
Journal of Southern Medical University
; (12): 960-964, 2011.
Article
en Zh
| WPRIM
| ID: wpr-332507
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of transfusion of apoptotic and necrotic thymocytes prior to sepsis on the survival rate of mice.</p><p><b>METHODS</b>BALB/c mice are divided into 3 groups and received intravenous injection of PBS (control), apoptotic thymocytes, or necrotic thymocytes. Three days later, cecal ligation and puncture (CLP) were performed to induce sepsis in these mice, and their survival and organ damage were observed.</p><p><b>RESULTS</b>The survival rates of mice in PBS group was 44.6% at the end of first week after CLP, and obvious lung and kidney damages were observed. A significant increase in the survival rate was found in apoptotic cell transfusion group (69.6%, P=0.012), with also lessened lung and kidney damages. The survival rate of mice in necrotic cell transfusion group was only 31.6% at 2 weeks, significantly lower than that in PBS group (P=0.035), and the lung and kidney damage was even more obvious.</p><p><b>CONCLUSION</b>Transfusion of apoptotic thymocytes 3 days before induction of sepsis can reduce organ damage and improve the survival rate of mice, while necrotic cell transfusion produces the opposite effect.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Terapéutica
/
Timo
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Tasa de Supervivencia
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Mortalidad
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Apoptosis
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Transfusión de Linfocitos
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Sepsis
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Biología Celular
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Modelos Animales de Enfermedad
/
Ratones Endogámicos BALB C
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Article