Molecular characterization and sequence comparison of the M and S segments of Hantavirus ZJ5 strain / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 434-436, 2008.
Article
en Zh
| WPRIM
| ID: wpr-332475
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>In order to understand the molecular characters of Hantavirus ZJ5 strain, its complete M and S genome were sequenced and compared with that of other hantavirus strains.</p><p><b>METHODS</b>We prepared the total RNA from ZJ5. Infected cells and the raw or purified RT-PCR product was cloned and sequenced.</p><p><b>RESULTS</b>With sequence compation, we found ZJ5 strain complete M and S segment had higher homology with SEO-type strains than other type of HV, but differential genes were 11.7%-19.2% and 6.7%-14.5% from SEOV. The phylogenetic trees constructed by complete M ind S segment showed that ZJ5 strain was located in SEOV group, and structured alone embranchment.</p><p><b>CONCLUSION</b>The ZJ5 strain was believed to belong to SEO-type virus,and suggest that ZJ5 strain is a new subtype S SEOV group,and structured alone embranchment.</p><p><b>CONCLUSION</b>The ZJ5 strain was believed to belong to SEO-type virus, and suggest that ZJ5 strain is a new subtype from other SEO viruses.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Filogenia
/
Virología
/
ADN Viral
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Secuencia de Aminoácidos
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Sitios de Estimulación de Linfocito Menor
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Orthohantavirus
/
Análisis de Secuencia de ADN
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Article