Construction of antisense RNA expression plasmid for u-PAR and its transfection to highly invasive PC-3M cell subclones / 华中科技大学学报（医学）（英德文版）
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 369-372, 2003.
Article
en En
| WPRIM
| ID: wpr-330903
Biblioteca responsable:
WPRO
ABSTRACT
To evaluate the specific inhibition of antisense u-PAR on the u-PAR expressions in highly invasive cell subclones and to determine its blocking function in the invasion by those cells, a cDNA fragment of u-PAR obtained by RT-PCR was inserted into a plasmid vector named pcDNA3 in antisense orientation. Then the antisense u-PAR recombinant was transfected into highly invasive cell subclones. The u-PAR expression in neo-resistant cells was examined by RT-PCR and immunohistochemical assay. Compared to the control cells, the content of mRNA and protein of u-PAR in transfected cells decreased sharply, and the rate of inhibition was 53% and 73%, respectively, indicating that an antisense u-PAR might have played a specific inhibitory role in its expression in the cells, which may provide a good cell model for making further investigation of the inhibitory effects of the antisense u-PAR on invasion in highly invasive cell subclones of human prostate carcinoma.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Plásmidos
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Neoplasias de la Próstata
/
Transfección
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Activador de Plasminógeno de Tipo Uroquinasa
/
ARN sin Sentido
/
Clonación Molecular
/
Receptores de Superficie Celular
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Línea Celular Tumoral
Límite:
Humans
/
Male
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Año:
2003
Tipo del documento:
Article