Identification of cattail pollen (puhuang), pine pollen (songhuafen) and its adulterants by ITS2 sequence / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 2189-2193, 2014.
Article
en Zh
| WPRIM
| ID: wpr-330323
Biblioteca responsable:
WPRO
ABSTRACT
DNA barcoding method was conducted for the authentication of pollen materials due to difficulty of discriminating pollen materials bearing morphological similarity. In this study, a specific focus was to identify cattail pollen (Puhuang) and pine pollen (Songhuafen) samples from their adulterants which are frequently mixed-together. Regions of the internal transcribed spacer (ITS2) from 60 samples were sequenced, and new primers for cattail pollen were designed according to the sequence information. The results from the NJ trees showed that the species of pine pollen, Puhuang and their adulterants can be classified as obvious monophyly. Therefore, we propose to adapt DNA barcoding methodology to accurately distinguish cattail pollen, pine pollen and their adulterant materials. It is a great help for drug regulatory agency to supervise the quality of medicinal materials.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Filogenia
/
Polen
/
Control de Calidad
/
Medicamentos Herbarios Chinos
/
Datos de Secuencia Molecular
/
Química
/
China
/
Contaminación de Medicamentos
/
Clasificación
/
ADN de Plantas
Tipo de estudio:
Diagnostic_studies
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2014
Tipo del documento:
Article