Study on identification of "Digeda" raw materials in Mongolian patent medicine by PCR amplification of specific alleles / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 793-798, 2015.
Article
en Zh
| WPRIM
| ID: wpr-330231
Biblioteca responsable:
WPRO
ABSTRACT
To explore a new method for identification of Mongolian patent medicine (MPM) by PCR amplification of specific alleles. Eight kinds of MPM were used to study the identification of "Digeda" raw materials. The total DNA of Lomatogonium rotatum and Corydalis bungeana samples were extracted through modified CTAB method, psbA-trnH sequence was amplified by PCR and sequenced directionally. Specific primer was designed. The DNA of 8 kinds of MPM also was extracted and purified by the commercial DNA purification kits. The rbcL and two pair of specific primers sequences were amplified. The specific amplified products were sequenced in forward directions. All specific sequences were aligned and were analyzed. The results indicated that L rotatum can be identified by specific primers from Digeda-4 Tang, Digeda-8 San, Digeda-4 San, and C. bungeana medicinal materials can be identified by specific primers from Li Dan Ba Wei San, Yi He Ha Ri-12 and A Ga Ri-35. PCR amplification of specific alleles can stably and accurately distinguish raw medicinal materials in MPM.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plantas Medicinales
/
Datos de Secuencia Molecular
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Reacción en Cadena de la Polimerasa
/
Clasificación
/
Cartilla de ADN
/
ADN de Plantas
/
Alelos
/
Medicina Tradicional Mongoliana
/
Genética
/
Métodos
Tipo de estudio:
Diagnostic_studies
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2015
Tipo del documento:
Article