Effect of high-fat or high-glucose diet on obesity and visceral adipose tissue in mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 614-619, 2014.
Article
en En
| WPRIM
| ID: wpr-329774
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of high-fat or high-glucose diet on obesity and visceral adipose tissue in C57BL/6 mice.</p><p><b>METHODS</b>Four-week-old C57BL/6 mice were allocated into normal diet group,high-fat diet group,and high-glucose diet group according to the random number table until 20 weeks old. Body weight,epididymal adipose tissue weight,blood leptin,fat infiltration in liver,M1/M2 macrophage subtypes,and monocyte chemoattractant protein-1 mRNA in epididymal adipose tissues were measured.</p><p><b>RESULTS</b>Compared with normal diet group,body weight,epididymal adipose tissue weight,and leptin concentration in high fat diet group at 20 weeks were significantly increased (P < 0.05),and oil red O staining showed more prominent adipocyte infiltration in liver in high-fat diet group than those in normal diet and high-glucose diet group. However,no apparent differences were seen in high-glucose diet group at 20 weeks in terms of body weight,epididymal adipose tissue weight and leptin concentration. In high-fat diet group,the macrophages infiltration in epididymal adipose tissue increased with time and the percentage of M2 macrophage decreased in high-fat diet group than that in high-glucose diet group(P<0.05). Compared with normal diet group,monocyte chemoattractant protein-1 mRNA expression increased significantly in high-fat diet group(P<0.05). In high-glucose group,however,no significant differences were discerned (P > 0.05).</p><p><b>CONCLUSION</b>High-fat diet,rather than 60% high glucose diet,will lead to obesity and macrophage infiltration in adipose tissues.</p>
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Base de datos:
WPRIM
Asunto principal:
Peso Corporal
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ARN Mensajero
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Tejido Adiposo
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Adipocitos
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Quimiocina CCL2
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Leptina
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Grasa Intraabdominal
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Dieta Alta en Grasa
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Genética
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Glucosa
Límite:
Animals
Idioma:
En
Revista:
Acta Academiae Medicinae Sinicae
Año:
2014
Tipo del documento:
Article