Troglitazone suppresses ICAM-1 and MMP-9 expressions by up-regulating PPARγ in HeLa cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1693-1701, 2014.
Article
en Zh
| WPRIM
| ID: wpr-329219
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of troglitazone, a proliferator-activated receptor-γ (PPARγ) agonist, on the expressions of intercellular adhesion molecule-1 (ICAM-1) and matrix metalloproteainse-9 (MMP-9) and cell proliferation in HeLa cells.</p><p><b>METHODS</b>MTT assay was used to measure the cell viability at different time points (0, 24, 48 and 72 h) after exposure to troglitazone. RT-PCR and Western blotting were employed to detect the mRNA and protein expressions of ICAM-1, MMP-9 and PPARγ in the cells. Electrophoretic mobility shift assay (EMSA) was performed to assess the changes in DNA binding activity of PPARγ.</p><p><b>RESULTS</b>The viability of HeLa cells were time-dependently inhibited by troglitazone, which also significantly reduced the mRNA and protein expressions of ICAM-1 and MMP-9 and increased PPARγ expression. The effects of troglitazone in inhibiting the cell viability and reducing ICAM-1 and MMP-9 expressions were antagonized by the application of the PPARγ antagonist GW9662. The result of EMSA also showed significantly increased DNA binding activity of PPARγ in the cells exposed to troglitazone.</p><p><b>CONCLUSION</b>The PPARγ agonist troglitazone can inhibit the expression of ICAM-1 and MMP-9 in HeLa cells by up-regulating PPARγ.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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ARN Mensajero
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Células HeLa
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Regulación hacia Arriba
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Supervivencia Celular
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Regulación de la Expresión Génica
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Cromanos
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Molécula 1 de Adhesión Intercelular
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Metaloproteinasa 9 de la Matriz
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Tiazolidinedionas
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2014
Tipo del documento:
Article