Establishment and identification of mouse lymphoma cell line EL4 expressing red fluorescent protein / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 107-110, 2010.
Article
en Zh
| WPRIM
| ID: wpr-328563
Biblioteca responsable:
WPRO
ABSTRACT
This study was purposed to construct a lentiviral vector encoding red fluorescent protein (DsRed) and transfect DsRed into EL4 cells for establishing mouse leukemia/lymphoma model expressing DsRed. The bicistronic SIN lentiviral transfer plasmid containing the genes encoding neo and internal ribosomal entry site-red fluorescent protein (IRES-DsRed) was constructed. Human embryonic kidney 293FT cells were co-transfected with the three plasmids by liposome method. The viral particles were collected and used to transfect EL4 cells, then the cells were selected by G418. The results showed that the plasmid pXZ208-neo-IRES-DsRed was constructed successfully, and the viral titer reached to 10(6) U/ml. EL4 cells were transfected by the viral solution efficiently. The transfected EL4 cells expressing DsRed survived in the final concentration 600 microg/ml of G418. The expression of DsRed in the transfected EL4 cells was demonstrated by fluorescence microscopy and flow cytometry. In conclusion, the EL4/DsRed cell line was established successfully.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Transfección
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Lentivirus
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Línea Celular Tumoral
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Citometría de Flujo
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Vectores Genéticos
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Genética
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Proteínas Luminiscentes
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Linfoma
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Ratones Endogámicos C57BL
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Article