Comparison of effect between homologous recombinant gene knockout and siRNA gene silence in cell lines / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 122-126, 2010.
Article
en Zh
| WPRIM
| ID: wpr-328560
Biblioteca responsable:
WPRO
ABSTRACT
The objective of this study was to compare the effects between knocking-out Sam68 gene by homologous recombination method and silencing the gene by siRNA silencing technique in DT40 cell line. Gene targeting technique was used to isolate Sam68 gene-deleted chicken DT40 cells. Meanwhile, Sam68 gene silencing cells was obtained by using stable expression of siRNA plasmid pSilencer-Sam68. Then, the function of these two cell lines were analyzed by comparing with wild-type DT40 cell line. The results showed that the growth retardation in Sam68 gene knocked-out cell line was observed due to elongation of the G2/M phase, but which could not be found in Sam68 gene silencing cell line. It is concluded that in accordance with study of protein function in living cells, use of gene knockout technique for cell line can provide the experimental results more real than those resulting from gene silence technique.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plásmidos
/
Transfección
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Regulación Neoplásica de la Expresión Génica
/
Pollos
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Marcación de Gen
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Silenciador del Gen
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ARN Interferente Pequeño
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Línea Celular Tumoral
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Técnicas de Inactivación de Genes
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Genética
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Article