Cerulenin changes apoptosis related genes expression in multiple myeloma cell line U266 / 中华血液学杂志
Chinese Journal of Hematology
; (12): 239-242, 2007.
Article
en Zh
| WPRIM
| ID: wpr-328347
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression changes of apoptosis related genes induced by cerulenin in multiple myeloma cell line U266 and explore its molecular mechanism.</p><p><b>METHODS</b>The expression changes of 96 apoptosis related genes were analyzed by superArray cDNA in U266 cells treated with cerulenin (20 microg/ml) for 12 h. Semi-quantitative RT-PCR was used to confirm the representative expression changes genes, Rip2, caspase 9 and TRAF2.</p><p><b>RESULTS</b>After treated with cerulenin for 12 h, 44 apoptosis related genes expression in the U266 cells were changed, among which 41 were over 2 fold increase and 3 over 2 fold decrease. The expression of caspase 9 was increased markedly, indicating that mitochondria pathway played a key role in cerulenin inducing apoptosis and TRAF2 expression change suggested that nuclear factor (NF) participates in cerulenin inducing apoptosis.</p><p><b>CONCLUSION</b>The death acceptor signaling pathway and the death acceptor non-dependence signaling pathway co-regulate cerulenin inducing apoptosis in U266 cells. Mitochondria pathway played the key role and nuclear factor (NF) participates in the apoptosis process.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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Transducción de Señal
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Regulación Neoplásica de la Expresión Génica
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Cerulenina
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Apoptosis
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Perfilación de la Expresión Génica
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Línea Celular Tumoral
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Genética
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Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2007
Tipo del documento:
Article