Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 99-103, 2005.
Article
en Zh
| WPRIM
| ID: wpr-327124
Biblioteca responsable:
WPRO
ABSTRACT
DNA was extracted from the peripheral venous blood of 338 subjects using BLOOD DNA MINI KIT. The 5 site SNP in 3 subtypes of Beta-AR were genotyped by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and allele-specific primer PCR techniques. The genotypes combination distribution of SNP at 5 sites in the 3 subtypes of Beta-AR were determined by clustering analysis technique. The natural combination distribution characteristics for SNP at 5 sites in the 3 subtypes of Beta-AR in 338 subjects were obtained. Sixty-seven combinations types were found. The preceding 5 combinations in the natural combination distribution of the SNP were: (1) The genotype combination of forty subjects was B1-AR S/S49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 11.83%. (2) The genotype combination of thirty-three subjects was B1-AR S/S49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/Q27+B3-AR W/W64, its probability was 9.76%. (3) The genotype combination of nineteen subjects was B1-AR S/S49+B1-AR R/G389+B2-AR R/G16+B2-AR Q/Q27+B3-AR W/W64, its probability was 5.62%. (4) The genotype combination of sixteen subjects was B1-AR S/S49+B1-AR R/G389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 4.74%. (5) The genotype combination of thirteen subjects was B1-AR S/G49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 3.85%. The obvious correlations exist among full sample and female or male subgroup, and between female and male subgroups.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Reacción en Cadena de la Polimerasa
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Receptores Adrenérgicos beta
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Clasificación
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Receptores Adrenérgicos beta 2
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Receptores Adrenérgicos beta 1
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Polimorfismo de Nucleótido Simple
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Receptores Adrenérgicos beta 3
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Genética
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Genotipo
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Métodos
Límite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2005
Tipo del documento:
Article