Study on a putative, proprotein convertase-cleaved product of HBV core protein in vitro / 中华肝脏病杂志
Zhonghua ganzangbing zazhi
; Zhonghua ganzangbing zazhi;(12): 595-598, 2010.
Article
en Zh
| WPRIM
| ID: wpr-326289
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the cleavage of HBV core protein in vivo by proprotein convertase furin or its family members and observe the intracellular localization of the putative cleaved product.</p><p><b>METHODS</b>Recombinant HBV core protein was incubated with furin under different conditions in vitro, and the reaction was checked with Western blotting. The recombinant vectors expressed the putative cleaved fragment and intact core protein (serves as control) were constructed. The stable expression cell lines were established by transfecting constructs into HepG2 cell line, for which indirect immunofluorescence staining was used by monoclonal anti-HBc against the region shared by core protein and its cleaved product .The confocal microscopy was carried out to observe the intracellular distribution.</p><p><b>RESULTS</b>HBV core protein was cleaved by furin in vitro under different tested conditions. The molecular weight of the major cleaved product just about 15,000 was in concordance with the expectation. The expressed cleaved fragment could react to the monoclonal antibody against core protein, and mainly located in cytosol in particle style just like the intact core protein.</p><p><b>CONCLUSION</b>HBV core protein can be cleaved by furin in vitro. The major cleaved product has similar antigenicity and subcellular distribution to core protein. These data suggest that proprotein convertase furin or its family members play important roles in HBV replication regulation, and the cleaved product may be involved in antiviral immunity of HBV infection. Further investigations are imperative.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Fisiología
/
Replicación Viral
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Transfección
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Virus de la Hepatitis B
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Microscopía Confocal
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Furina
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Proproteína Convertasas
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Microdisección
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Células Hep G2
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Vectores Genéticos
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua ganzangbing zazhi
Año:
2010
Tipo del documento:
Article