Effect of matrine and oxymatrine on proliferation and expression of Stat3 and Stat5 in SMMC-7721 cell line / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 2234-2237, 2008.
Article
en Zh
| WPRIM
| ID: wpr-324859
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of matrine and oxymatrine on proliferation and the expression of Stat3, Stat5 mRNA in SMMC-7721 cell line.</p><p><b>METHOD</b>Treated with matrine and oxymatrine, the inhibitory effect on SMMC-7721 cell proliferation was detected by MTT, double fluorescence labeling was applied to measure the apotosis ratios of SMMC-7721cells, the expression of Stat3 and Stat5 mRNA in SMMC-7721 cell line were assessed with RT-PCR.</p><p><b>RESULT</b>Matrine and oxymatrine could inhibit the proliferation and induce the apoptosis of SMMC-7721 cells and it was time and dose dependent, the expression of Stat3 and Stat5 mRNA in SMMC-7721 cell with matrine and oxymatrine were significantly lower than those in control group (P<0.05 or P<0.01). Compared with the same dose of matrine and oxymatrine, matrine showed stronger effect on cell proliferation, apoptosis, and Stat3 and Stat5 mRNA (P<0.05 or P<0.01).</p><p><b>CONCLUSION</b>Matrine and oxymatrine inhibited the proliferation and induced the of SMMC-7721 cells significantly, the mechanism of which might be related to the down-regulation of stat3 and stat5 mRNA and inhibition of the signaling transduction pathway.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Quinolizinas
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Medicamentos Herbarios Chinos
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Regulación de la Expresión Génica
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Apoptosis
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Línea Celular Tumoral
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Proliferación Celular
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Alcaloides
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Factor de Transcripción STAT3
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2008
Tipo del documento:
Article