Pilot-scale production of recombinant plasmid pUDK-HGF / 生物工程学报
Chinese Journal of Biotechnology
; (12): 247-252, 2011.
Article
en Zh
| WPRIM
| ID: wpr-324556
Biblioteca responsable:
WPRO
ABSTRACT
pUDK-HGF, the recombinant plasmid DNA encoding human hepatocyte growth factor (HGF), can treat ischaemic disease. A great quantity of pharmaceutical pUDK-HGF is needed. A pilot-scale production process of pUDK-HGF was established based on a new chromatographic media (plasmidselect), including fermentation, cell harvesting, alkaline lysis, ultrafiltration, RNA removing and buffer exchanging on Sephacryl S-1000, capturing supercoiled plasmid DNA with plasmidselect, and removing the salt with Sepharose 6BFF. The process does not use RNase enzyme and toxic solvents.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plásmidos
/
ADN Recombinante
/
ADN Superhelicoidal
/
Proyectos Piloto
/
Factor de Crecimiento de Hepatocito
/
Escherichia coli
/
Fermentación
/
Vectores Genéticos
/
Genética
/
Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Article