Development of a killed but metabolically active anthracis vaccine candidate strain / 生物工程学报
Chinese Journal of Biotechnology
; (12): 781-789, 2011.
Article
en Zh
| WPRIM
| ID: wpr-324538
Biblioteca responsable:
WPRO
ABSTRACT
Anthrax is a zoonosis caused by Bacillus anthracis, which seriously affects human health. In recent years, a special phenomenon is found that the metabolic active of a bacterium remains after it is killed. To development of a KBMA (killed but metabolically active) Bacillus anthracis vaccine candidate strain, a plasmid pMAD and a recombinase system Cre-loxP were used to knockout the uvrAB gene of B. anthracis AP422 which lacks both of two plasmids pXO1 and pXO2. The results of PCR and RT-PCR shows that uvrAB genes were deleted from B. anthracis AP422 chromosome successfully. The constructed B. anthracis AP422deltauvrAB was inactivated by photochemical treatment (PCT) including an exposure in a long-wave-length ultraviolet (UVA) light and a treatment of 8-Methoxypsoralen (8-MOP), then the metabolic activity were detected by the method of MTS. The results showed that the killed B. anthracis AP422deltauvrAB maintained a highly metabolic activity for at least 4 hours, showing a state of KBMA. The KBMA strain of B. anthracis AP422deltauvrAB provides the prospective vaccine candidate strain for anthrax.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Efectos de la Radiación
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Bacillus anthracis
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Rayos Ultravioleta
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Vacunas de Productos Inactivados
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Vacunas contra el Carbunco
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Alergia e Inmunología
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Técnicas de Inactivación de Genes
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Genética
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Metoxaleno
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Article