Preparation and application of a colloidal gold strip to detect the rabies antibody / 生物工程学报
Chinese Journal of Biotechnology
; (12): 799-804, 2011.
Article
en Zh
| WPRIM
| ID: wpr-324536
Biblioteca responsable:
WPRO
ABSTRACT
To develop a specific, rapid, and convenient immunochromatography assay (ICA) to detect the rabies antibody in clinical sample from immuned dogs by rabies vaccine. Colloidal gold particles labeled with purified rabies virus (CVS11) were used as the detector reagent. The staphylococcal protein A (SPA) and pured rabbit anti-rabies virus IgG were blotted on the test and control regions of nitrocellulose membrane. Then the strip was assembled with sample pad, absorbing pad, and dorsal shield. The assay samples (261 dog's serum) were collected from Wildlife Rabies Disease Diagnostic Laboratories of Ministry of Agriculture in China, Institute of Military Veterinary, Academy of Military Medical Sciences and other six provinces, including rabies virus positive and negative serum. The performance of the strip was compared to fluorescent antibody virus neutralization test. The neutralizing antibody titer could be detected above 0.5 IU. The strip did not change of performance when stored at room temperature for 12 months. It may offer reference of neutralizing antibody titer level after dogs immuned rabies vaccine and determin whether the dogs need to be immuned again.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Rabia
/
Virus de la Rabia
/
Tiras Reactivas
/
Sangre
/
Vacunas Antirrábicas
/
Cromatografía de Afinidad
/
Sensibilidad y Especificidad
/
Vacunación
/
Oro Coloide
/
Alergia e Inmunología
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Article