Study on oral absorption enhancers of astragalus polysaccharides / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 1243-1247, 2014.
Article
en Zh
| WPRIM
| ID: wpr-321330
Biblioteca responsable:
WPRO
ABSTRACT
Astragalus polysaccharides was lounded to 4-(2-aminoethylphenol), followed by labeling the APS-Tyr with fluorescein-5-isothiocyanate (FITC) at the secondary amino group. The absorption enhancement effects of low molecular weight chitosan and protamine on astragalus polysaccharides were evaluated via Caco-2 cell culture model. The results show that the fluorecent labeling compound has good stability and high sensitivity. On the other hand low molecular weight chitosan and protamine also can promoted absorption of the astragalus polysaccharides without any cytotoxity, and the absorption increase was more significant with increasing the amount of low molecular weight chitosan and protamine. At the same time, the low molecular weight chitosan has slightly better effect. The transepithelial electric resistance (TEER) of Caco-2 cells show that absorption enhancers could improve its membrane transport permeability by opening tight junctions between cells and increasing the cell membrane fluidity.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Polisacáridos
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Transporte Biológico
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Farmacocinética
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Extractos Vegetales
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Química
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Fluoresceína-5-Isotiocianato
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Células CACO-2
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Planta del Astrágalo
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Absorción
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Colorantes Fluorescentes
Límite:
Humans
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2014
Tipo del documento:
Article