Expression analyses of BcUGT3 and BcUGT6, and their in vitro expression in Escherichia coli / 中国中药杂志
Zhongguo Zhong Yao Za Zhi
; (24): 185-191, 2014.
Article
en Zh
| WPRIM
| ID: wpr-319631
Biblioteca responsable:
WPRO
ABSTRACT
The tissue-specific and MeJA-induced transcriptional levels of BcUGT3 and BcUGT6 in Bupleurum chinense were analyzed in the present study. The transcriptional levels of BcUGT3 in root, leaf, flower and fruit were similar and they all were higher than those in stem. The transcriptional level of BcUGT6 was the highest in leaf and the lowest in flower among in all tested tissues. With non-treated adventitious roots as control, BcUGT6's transcriptional levels were elevated to nearly 2 folds for 2 h, 8 h, 24 h, 2 d and 4 d in MeJA-treated adventitious roots of B. chinense. It showed that the transcriptional level of BcUGT6 was slightly affected by MeJA. While, BcUGT3's transcriptional levels were gradually elevated, and till 4 d after MeJA treatment, the expression level was about 7 folds than that of non-treated control. Using pET-28a (+), the expressions of two genes was investigated. Induced by IPTG, the target proteins were expressed in E. coli and then purified. All the results obtained in the present study will be helpful for follow-up bio-function analysis of BcUGT3 and BcUGT6.
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1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Transcripción Genética
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Señales de Clasificación de Proteína
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Expresión Génica
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Membrana Celular
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Química
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Análisis de Secuencia
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Estructura Secundaria de Proteína
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Regulación de la Expresión Génica de las Plantas
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Ciclopentanos
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Año:
2014
Tipo del documento:
Article