Recombinant human bone morphogenetic protein-2 down-regulates LASP1 and up-regulates ferritin during osteogenic differentiation of beagle dog bone marrow mesenchymal stem cells / 南方医科大学学报
Journal of Southern Medical University
; (12): 1207-1212, 2013.
Article
en Zh
| WPRIM
| ID: wpr-319445
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify the proteins involved in osteogenic differentiation of beagle dog bone marrow mesenchymal stem cells (BMSCs) and explore its possible regulation mechanism of osteogenic differentiation of BMSCs.</p><p><b>METHODS</b>Cultured beagle dog BMSCs were induced by recombinant human bone morphogenetic protein-2 (rhBMP-2) for 7 days. The differentially expressed proteins between cells with osteogenic differentiation and control cells were identified by proteomics analysis based on two-dimensional gel electrophoresis. Q-PCR and Western blotting were used to verify the interested protein of LASP1, ferritin light chain and heavy chain.</p><p><b>RESULTS</b>Osteogenic differentiation was induced successfully in the BMSCs. Twenty differentially expressed proteins were identified by proteomic analysis, including 9 down-regulated and 11 up-regulated ones. Q-PCR and Western blotting demonstrated a significant reduction of LASP1 expression and significant up-regulation of ferritin in the BMSCs after a 7-day induction with rhBMP-2.</p><p><b>CONCLUSION</b>LASP1, which plays an important role in the regulation of the activity of the cytoskeleton, and ferritin, an important molecule in cellular iron homeostasis, can be critical in the osteogenic differentiation of beagle dog BMSCs induced by rhBMP-2.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Osteogénesis
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Farmacología
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Proteínas Recombinantes
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Células de la Médula Ósea
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Regulación hacia Abajo
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Diferenciación Celular
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Regulación hacia Arriba
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Células Cultivadas
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Factor de Crecimiento Transformador beta
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Biología Celular
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2013
Tipo del documento:
Article