Prokaryotic expression of HLA-B * 2705 heavy chain and identification for its activity / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1028-1031, 2007.
Article
en Zh
| WPRIM
| ID: wpr-318796
Biblioteca responsable:
WPRO
ABSTRACT
In order to investigate the expression of heavy chain of HLA-B * 2705 in prokaryotic system and identify its activity, the extra-membrane gene fragment of HLA-B * 2705 was amplified from full-length HLA-B*2705 cDNA by PCR and cloned into pGEM-T vector. After identification by sequencing, the prokaryotic expressing vector pET32a (+)-B * 2705 was constructed. The antigenic activity of expressed protein was identified by Western blot and antibody blocking reaction. The results indicated that the fused HLA-B * 2705 protein expression with high efficiency was obtained. The expressed product was more than 50% of the total bacteria protein. The antigenic activity of expressed protein was confirmed by Western blot and antibody blocking reaction. It is concluded that HLA-B * 2705 fusion protein are obtained as basis for the further studies.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Antígeno HLA-B27
/
Clasificación
/
Cadenas Pesadas de Inmunoglobulina
/
Alergia e Inmunología
/
Escherichia coli
/
Vectores Genéticos
/
Genética
/
Metabolismo
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2007
Tipo del documento:
Article