Expression and purification of EBV-LMP2 protein / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 168-170, 2010.
Article
en Zh
| WPRIM
| ID: wpr-316934
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To obtain a second Epstein-Barr virus membrane protein (LMP2) in insect cells.</p><p><b>METHODS</b>The full length EBV-LMP2 gene was inserted into baculovirus expression transfer vector pFastBac HT B to obtain the recombinant baculoviruses Bac-LMP2. And generation of recombinant baculoviruses was followed by transfection of the recombinant Bac-LMP2 into insect cells, then the recombinant LMP2 protein was recognized by SDS-PAGE and western blot. The expressed LMP2 protein was purified by one step with Ni-NTA metal chelation chromatography.</p><p><b>RESULTS</b>The expressed LMP2 protein was confirmed by SDS-PAGE and western blot. The purity of purified LMP2 protein is up to 86% by HPLC analysis.</p><p><b>CONCLUSION</b>The EBV-LMP2 was expressed in insect cells, and the purified LMP2 protein was obtained.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Virulencia
/
Proteínas Recombinantes
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Proteínas de la Matriz Viral
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Western Blotting
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Baculoviridae
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Herpesvirus Humano 4
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Biología Celular
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Electroforesis en Gel de Poliacrilamida
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Vectores Genéticos
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Genética
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2010
Tipo del documento:
Article