Study on the injury of vascular endothelial cells affected by dust particles from workplaces in pottery factories and tungsten mines / 中华劳动卫生职业病杂志
Zhonghua laodong weisheng zhiyebing zazhi
; Zhonghua laodong weisheng zhiyebing zazhi;(12): 735-739, 2010.
Article
en Zh
| WPRIM
| ID: wpr-313550
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>to assess direct adverse effects of occupational dusts from pottery factories and tungsten mines on vascular endothelial cells in vitro test.</p><p><b>METHODS</b>human umbilical vein endothelial cell (HUVEC) line HUV-EC-C were used as target cells. HUVEC were then treated with respirable dust particles from workplaces in pottery factories and tungsten mines in concentrations of 25, 50, 100, 200 and 400 microg/ml for 24 h. Standard quartz was used as control. LDH activity, cell viability, the release of NO and TNF-α levels were determined to assess the biological responses of the dust particles.</p><p><b>RESULTS</b>dose-response relationships between the dust concentrations and the enhancement of the LDH activity, the release of NO and TNF-α were found in both dust particles from pottery factories and tungsten mines. The cell viability decreased with the increase of dust concentration from 25 to 400 microg/ml. Compared with the dust particles from workplaces, the quartz dust induced significantly higher LDH activity (P < 0.05) after cultured with HUVEC. No significant difference of releases of NO were observed among the dust particles from workplaces and standard quartz. However, significantly higher levels of TNF-α were induced by standard quartz compared with dust samples from workplaces at concentrations of 200, 400 microg/ml.</p><p><b>CONCLUSION</b>occupational dust particles from workplaces and quartz could induce the injury and the releases of TNF-α from HUVEC.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Cuarzo
/
Tungsteno
/
Células Cultivadas
/
Cerámica
/
Exposición Profesional
/
Factor de Necrosis Tumoral alfa
/
Polvo
/
Células Endoteliales de la Vena Umbilical Humana
/
Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua laodong weisheng zhiyebing zazhi
Año:
2010
Tipo del documento:
Article