Effect of ventricle injection of Nogo-A antibody on neuronal regeneration following hypoxic-ischemic brain damage in the neonatal rat / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 301-304, 2007.
Article
en Zh
| WPRIM
| ID: wpr-312713
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>Nogo-A antibody IN-1 can neutralize Nogo-A, a neurite growth inhibitory protein, promoting axonal regeneration following lesions of the central nervous system (CNS) in adult rats. This study aimed to examine the effect of ventricle injection of Nogo-A antibody on neuronal regeneration in neonatal rats following hypoxic-ischemic brain damage (HIBD).</p><p><b>METHODS</b>A model of neonatal HIBD was prepared by the ligation of the left common carotid artery, followed by 8% hypoxia exposure. Forty HIBD rats were randomly given a ventricle injection of 10 microL Nogo-A antibody IN-1 (IN-1 group) or 10 microL artificial cerebrospinal fluid (artificial CSF group) (n=20 each). Another 20 neonatal rats were sham-operated, without hypoxia-ischemia, and were used as the controls. The levels of Nogo-A and GAP-43 protein in the brain were measured by immunohistochemistry.</p><p><b>RESULTS</b>The number of immunohistory positive cells of Nogo-A in the brain in the IN-1 group (28.61+/-1.70) was obviously less than that in the artificial CSF (39.52 +/-1.40) and the sham-operated groups (32.78 +/- 1.87) (both P < 0.01). There were significant differences in the Nogo-A protein expression between the artificial CSF and the sham-operated groups (P < 0.01). The GAP-43 protein expression in the IN-1 group (31.14 +/- 1.88) was noticeably higher than that in the artificial CSF group (27.73 +/- 1.43 ) (P < 0.01). Both the IN-1 and the artificial CSF groups showed lower GAP-43 protein levels than the sham-operated groups (33.64 +/- 1.24) (both P < 0.01).</p><p><b>CONCLUSIONS</b>Nogo-A antibody can reduce the expression of Nogo-A protein in the brain and thus promote neuronal regeneration in neonatal rats following HIBD. An increased GAP-43 protein expression in the brain after Nogo-A antibody administration shows an enhanced neuronal regeneration in the neonatal rats following HIBD.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Terapéutica
/
Química Encefálica
/
Inmunohistoquímica
/
Ratas Sprague-Dawley
/
Proteína GAP-43
/
Hipoxia-Isquemia Encefálica
/
Alergia e Inmunología
/
Proteínas Nogo
/
Inyecciones Intraventriculares
/
Animales Recién Nacidos
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2007
Tipo del documento:
Article