Microwave-induced Apoptosis and Cytotoxicity of NK Cells through ERK1/2 Signaling / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 323-332, 2017.
Article
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| WPRIM
| ID: wpr-311408
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate microwave-induced morphological and functional injury of natural killer (NK) cells and uncover their mechanisms.</p><p><b>METHODS</b>NK-92 cells were exposed to 10, 30, and 50 mW/cm2 microwaves for 5 min. Ultrastructural changes, cellular apoptosis and cell cycle regulation were detected at 1 h and 24 h after exposure. Cytotoxic activity was assayed at 1 h after exposure, while perforin and NKG2D expression were detected at 1 h, 6 h, and 12 h after exposure. To clarify the mechanisms, phosphorylated ERK (p-ERK) was detected at 1 h after exposure. Moreover, microwave-induced cellular apoptosis and cell cycle regulation were analyzed after blockade of ERK signaling by using U0126.</p><p><b>RESULTS</b>Microwave-induced morphological and ultrastructural injury, dose-dependent apoptosis (P < 0.001) and cell cycle arrest (P < 0.001) were detected at 1 h after microwave exposure. Moreover, significant apoptosis was still detected at 24 h after 50 mW/cm2 microwave exposure (P < 0.01). In the 30 mW/cm2 microwave exposure model, microwaves impaired the cytotoxic activity of NK-92 cells at 1 h and down regulated perforin protein both at 1 h and 6 h after exposure (P < 0.05). Furthermore, p-ERK was down regulated at 1 h after exposure (P < 0.05), while ERK blockade significantly promoted microwave-induced apoptosis (P < 0.05) and downregulation of perforin (P < 0.01).</p><p><b>CONCLUSION</b>Microwave dose-dependently induced morphological and functional injury in NK-92 cells, possibly through ERK-mediated regulation of apoptosis and perforin expression.</p>
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Base de datos:
WPRIM
Asunto principal:
Efectos de la Radiación
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Células Asesinas Naturales
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Transducción de Señal
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Regulación hacia Abajo
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Ciclo Celular
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Línea Celular
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Apoptosis
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Sistema de Señalización de MAP Quinasas
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Relación Dosis-Respuesta en la Radiación
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Subfamilia K de Receptores Similares a Lectina de Células NK
Límite:
Humans
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2017
Tipo del documento:
Article