Expression of Wnt5a, APC, β-catenin and their clinical significance in human colorectal adenocarcinoma / 中华肿瘤杂志
Zhonghua zhong liu za zhi
; (12): 674-678, 2012.
Article
en Zh
| WPRIM
| ID: wpr-307318
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of Wnt5a gene mRNA and Wnt5a, APC, β-catenin proteins in human colorectal adenocarcinoma (CRC) and explore its clinical significance.</p><p><b>METHODS</b>Wnt5a mRNA level was measured in 30 patients with CRC and paired non-tumor tissues by real-time PCR. Immunohistochemical staining of Wnt5a, APC, β-catenin was performed in samples of 62 patients with CRC using SP system.</p><p><b>RESULTS</b>The relative expression level of Wnt5a mRNA in fresh CRC is 0.1232 ± 0.0140, which is significantly higher than that in adjacent colorectal mucosa (0.0497 ± 0.0074, P = 0.02). A low expression of Wnt5a protein was observed in 38 of 62 CRC. Wnt5a protein expression was closely correlated with the tumor types and the degree of tumor differentiation (P < 0.05). There was no apparent relationship with lymph node metastasis, depth of myometrial invasion and TNM stages (P > 0.05). APC protein was decreased in 38 of 62 CRC. The expression of APC was closely correlated with the tumor types (P < 0.05). There was no apparent relationship with the degree of tumor differentiation, lymph node metastasis, depth of myometrial invasion and TNM stages (P > 0.05). The expression of β-catenin was observed in cytoplasm and/or cell nuclei in 50 of 62 CRC. The positive rate of β-catenin expression was closely correlated with the degree of tumor differentiation, lymph node metastasis, depth of myometrial invasion and TNM stages (P < 0.05). There was no apparent relationship with the tumor types (P > 0.05). The expressions of Wnt5a (r = 0.271, P = 0.027) and APC (r = 0.343, P = 0.004) were correlated with that of β-catenin in CRC, respectively, but there was no correlation between the expressions of Wnt5a and APC protein (r = 0.218, P = 0.078) in the tumors.</p><p><b>CONCLUSIONS</b>Wnt5a, APC and β-catenin genes might be involved in the carcinogenesis and development of CRC. It is hypothesized that down-regulation of APC and Wnt5a proteins may be one of causes of ectopic expression of β-catenin in CRC.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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ARN Mensajero
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Inmunohistoquímica
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Neoplasias Colorrectales
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Adenocarcinoma
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Regulación hacia Abajo
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Diferenciación Celular
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Proteínas Proto-Oncogénicas
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Genes APC
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Carcinoma de Células en Anillo de Sello
Límite:
Adult
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Aged
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Aged80
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Zhonghua zhong liu za zhi
Año:
2012
Tipo del documento:
Article