Screening PIAS2-interacting proteins in the mouse using the yeast two-hybrid system / 中华男科学杂志
National Journal of Andrology
; (12): 775-780, 2011.
Article
en Zh
| WPRIM
| ID: wpr-305792
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen and identify PIAS2-interacting proteins from the mouse spermatogonial cDNA library using the yeast two-hybrid system, and to investigate the action mechanism of PIAS2 in spermatogenesis.</p><p><b>METHODS</b>With pGBKT7-PIAS2 as a bait plasmid, the positive clones interacting with pGBKT7-PIAS2 were screened from the mouse spermatogonial cDNA library, the inserted fragments were sequenced and underwent bioinformatic analysis, and their interaction was verified using the yeast two-hybrid system.</p><p><b>RESULTS</b>Through screening, sequencing, homology analysis and yeast two-hybrid verification, we obtained 8 different candidate proteins interacting with PIAS2, including Cyfip2, Psmb3, Nmel, nischarin, Ints10, Nsun5, Gnb211 and Ndufaf3.</p><p><b>CONCLUSION</b>Eight different genes were successfully obtained using the yeast two-hybrid system, and their encoding proteins interacted with PIAS2, which might be related with male fertility regulation. Our findings have offered some new clues to the action mechanism of PIAS2 in spermatogenesis.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Unión Proteica
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Espermatogénesis
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Espermatogonias
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Secuencia de Bases
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Biblioteca de Genes
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Técnicas del Sistema de Dos Híbridos
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Proteínas Inhibidoras de STAT Activados
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Dominios y Motivos de Interacción de Proteínas
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Genética
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Metabolismo
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
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Screening_studies
Límite:
Animals
Idioma:
Zh
Revista:
National Journal of Andrology
Año:
2011
Tipo del documento:
Article