Enhanced immune response of a novel T-cell immunogen in vaccine for foot-and-mouth disease / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1281-1291, 2011.
Article
en Zh
| WPRIM
| ID: wpr-304576
Biblioteca responsable:
WPRO
ABSTRACT
We investigated the enhanced immune response of a recombinant T cell immunogen as an effective cellular immune adjuvant. The T cell immunogen named TI contained several T cell epitopes from the VP1, VP4, 3A and 3D proteins of foot-and-mouth disease virus (FMDV) and two pan-T helper (T(H)) cell sites to broaden the immunogenicity of the protein. Meanwhile, another fusion protein named OA-VP1 was expressed in bacteria, which contained two VP1 proteins of O and Asia1 type FMDV. Mice were vaccinated with commercially inactivated vaccine or OA-VP1 protein with or without the TI immunogen. The results show that mice inoculated with inactivated vaccine or OA-VP1 protein supplemented with TI immunogen produced significantly higher level of neutralizing antibodies (P < 0.01 or P < 0.05) than the mice only inoculated with inactivated vaccine or OA-VP1 protein by microneutralization assay. An obvious increase in T cell number by flow cytometric analysis and significantly higher concentration of IFN-gamma secreted in culture media of spleen lymphocytes were observed in groups supplemented with TI immunogen (P < 0.01). TI immunogen was an effective stimulator for humoral and cellular immunity and could help improve the immunogenicity of inactivated vaccine or protein subunit vaccine.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Virología
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Vacunas Virales
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Adyuvantes Inmunológicos
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Inmunización
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Epítopos de Linfocito T
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Virus de la Fiebre Aftosa
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Proteínas de la Cápside
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Alergia e Inmunología
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Fiebre Aftosa
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Article