Role of Toll-like receptor 2 in primary immune thrombocytopenia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1033-1037, 2014.
Article
en Zh
| WPRIM
| ID: wpr-302353
Biblioteca responsable:
WPRO
ABSTRACT
The aim of this study was to explore the role of Toll-like receptor (TLR) 2 in primary immune thrombocytopenia (ITP) by detecting TLR2 expression in the peripheral blood lymphocytes of patients with ITP and evaluating the role of TLR2 activation on inflammatory cytokine secretion. A total of 39 ITP patients and 21 normal controls were enrolled in this study. The expression of TLR2 was detected by real-time PCR and flow cytometry, and the concentration of IL-6 and TNF-α in culture supernatant of PBMNC treated with pam3CSK4 for 48 hours were detected by ELISA. The results showed that the expression of TLR2 mRNA in active ITP patients (3.561 ± 0.741) was significantly higher than that in normal controls (1.750 ± 0.314) (P < 0.05), but there was no statistically significant difference between remission ITP patients (2.333 ± 0.448) and normal controls (P > 0.05) . Flow cytometry analysis found that the TLR2 was not expressed on T and B cells, but expressed on all monocytes both from ITP patients and normal controls. Further activation experiment showed that TLR2 activation in vitro could induce the expression of IL-6 (1644 ± 634.0 vs 4111 ± 525.2 pg/ml) and TNF-α (75.37 ± 22.31 vs 326.0 ± 109.9 pg/ml) in PBMNC from ITP patients (both P < 0.05), but just could promote IL-6 expression in normal controls (2119 ± 636.9 vs 4671 ± 315.9 pg/ml)(P < 0.05). It is concluded that the expression of TLR2 mRNA is up-regulated in PBMNC of ITP patients, and this increased TLR2 maybe participate in ITP through inducing secretion of inflammatory cytokines.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Trombocitopenia
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ARN Mensajero
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Estudios de Casos y Controles
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Células Cultivadas
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Interleucina-6
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Factor de Necrosis Tumoral alfa
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Alergia e Inmunología
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Receptor Toll-Like 2
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Genética
Tipo de estudio:
Observational_studies
Límite:
Adolescent
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Adult
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Aged
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Child
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2014
Tipo del documento:
Article