Expression of osteoclast differentiation factor and intercellular adhesion molecule-1 of bone marrow mesenchymal stem cells enhanced with osteogenic differentiation / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 240-243, 2005.
Article
en Zh
| WPRIM
| ID: wpr-300324
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investgate whether the expression of osteoclast differentiation factor (ODF), intercellular adhesion molecule-1 (ICAM-1) depended on the stage of osteoblastic differentiation from rat bone marrow mesenchymal stem cells (BMSC).</p><p><b>METHODS</b>BMSC (4 passage) were selected for osteogenic differentiation by treated with osteogenic supplements (OS). Cells were harvested by day 0, 3, 6, 9, 12, 18 respectively after osteogenic inducement. In each experiment, control and OS-treated cells were processed in parallel. ODF and ICAM-1 mRNA were analyzed by semiquantitave RT-PCR assay.</p><p><b>RESULTS</b>Expression of ODF was enhanced with osteogenic differentiation guadully. whereas, expression of ICAM-1 was activated at OS-treated day 6, then keeping at a stable level.</p><p><b>CONCLUSION</b>This study indicated that BMSC undergoing osteogenic inducement was an ideal model for studying the differentiation and maturation of osteoblasts. During the early stage of differentiation along osteoblasts from stem cells to osteocytes, BMSC or osteoprogenitor react somewhat differently from osteoblasts, suggesting the ability of osteoblasts to regulating differentiation and maturation of osteoclasts have been improved with osteogenic culture.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Osteoblastos
/
Osteoclastos
/
Osteogénesis
/
Células Madre
/
Células de la Médula Ósea
/
Diferenciación Celular
/
Células Cultivadas
/
Molécula 1 de Adhesión Intercelular
/
Ligando RANK
/
Células Madre Mesenquimatosas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
West China Journal of Stomatology
Año:
2005
Tipo del documento:
Article