AFLP analysis of genetic diversity of Prunella / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 260-263, 2009.
Article
en Zh
| WPRIM
| ID: wpr-298422
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the variety of the genetic polymorphism of eight Prunella germplasm resources by AFLP analysis.</p><p><b>METHOD</b>The amplified fragment length polymorphism (AFLP) tags were applied to screen out 32 selective amplification primer pairs, the amplified bands as original matrix were analyzed with NTSYS-PC software for the similarity between the Prunella germplasm and the construction of genetic phylogenetic tree.</p><p><b>RESULT</b>SDS extraction of genomic Prunella DNA showed a good quality, could meet the requirements of AFLP analysis. From 32 selective amplification primer pairs, 10 pairs with strong polymorphism, better band and higher resolution were used for the construction of the AFLP Prunella fingerprint, all eight Prunella germplasms were separated, they were divided into 3 categories.</p><p><b>CONCLUSION</b>Prunella germplasm resources are rich in genetic diversity, certain morphological characteristics and differences are associate with genotype.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Polimorfismo Genético
/
Variación Genética
/
Clasificación
/
ADN de Plantas
/
Prunella
/
Análisis del Polimorfismo de Longitud de Fragmentos Amplificados
/
Genética
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2009
Tipo del documento:
Article