Fusion expression of human renal cell carcinoma-associated antigen G250/MN/CA IX in prokaryotic expression system / 南方医科大学学报
Journal of Southern Medical University
; (12): 307-309, 2007.
Article
en Zh
| WPRIM
| ID: wpr-298179
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To achieve high expression of human renal cell carcinoma-associated antigen G250 in Escherichia coli.</p><p><b>METHODS</b>The gene fragments encoding the protein obtained by PCR was cloned into prokaryotic expression vector pET32a(+) and expressed in E. coli Rosseta. The immunogenicity of the recombinant protein was evaluated by Western blotting.</p><p><b>RESULTS</b>The plasmid pET32a(+)/G250 was constructed and expressed in E. coli Rosseta successfully. Western blot analysis showed that the recombinant protein could be specifically recognized by monoclonal antibody M75.</p><p><b>CONCLUSION</b>Efficient G250 expression is achieved in prokaryotic expression system, which may facilitate further functional study of the protein and its monoclonal antibody preparation.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Proteínas Recombinantes de Fusión
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Biomarcadores de Tumor
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Expresión Génica
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Western Blotting
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Clonación Molecular
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Anhidrasas Carbónicas
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Alergia e Inmunología
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Escherichia coli
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Anhidrasa Carbónica IX
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Genética
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2007
Tipo del documento:
Article