Quasispecies investigation on swine hepatitis E viruses / 病毒学报
Chinese Journal of Virology
; (6): 40-44, 2010.
Article
en Zh
| WPRIM
| ID: wpr-297920
Biblioteca responsable:
WPRO
ABSTRACT
The objective of current study was to investigate the quasispecies of hepatitis E virus in swine. The partial ORF2 region of HEV envelope gene from four swine HEV strains was amplified by RT-nested polymerase chain reaction (RT-nPCR). After cloning and transformation of PCR products, 20 positive clones of each HEV isolate were subject to sequencing and DNA analysis. The homology among the different clones of each isolates was 96.8%-99.7%, 98.8%-99.7%, 98.8%-99.7% and 100%, respectively, while there was 96.8%-100% sequence identity at the nucleotide level compared with HEV strains isolated in Shanghai (SAAS-JDY5). This study confirmed that there existed quasispecies of HEV in swine.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Filogenia
/
Porcinos
/
Virología
/
Reservorios de Enfermedades
/
Secuencia de Bases
/
Química
/
China
/
Sistemas de Lectura Abierta
/
Alineación de Secuencia
/
Secuencia de Aminoácidos
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2010
Tipo del documento:
Article