Expression of KISS-1 and GnRH in rat hypothalamus / 中华男科学杂志
Zhonghua nankexue
; Zhonghua nankexue;(12): 315-319, 2007.
Article
en Zh
| WPRIM
| ID: wpr-297734
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To assay the expression of KiSS-1 and GnRH in the male rat hypothalamus at different developmental stages, and to explore the significance of KiSS-1 in sex development onset and normal reproduction regulation.</p><p><b>METHODS</b>Expression analyses of KiSS-1 and GnRH genes were conducted in the rat hypothalamus at different developmental stages with RT-PCR and real time-PCR. The testosterone level was assayed by chemoluminescence technique.</p><p><b>RESULTS</b>KiSS-1 mRNA rose gradually during sex development in the rat hypothalamus, highest at puberty and lowered a little at adulthood. KiSS-1 mRNA of the prepubertal, early pubertal, pubertal and adult rats was 1.7, 2.1, 3.5 and 2.0 times higher than that of the infantile rats respectively. The expression of GnRH and KiSS-1 correlated positively (r = 0.905, P < 0.05). But the activation of GnRH neuron was later than KiSS-1. The expression of GnRH was the highest in the puberty rats. GnRH mRNA of the prepubertal, early pubertal, pubertal and adult rats was 1.1, 1.94, 2.42 and 1.92 times higher than that of the infantile rats respectively. The level of testosterone in the adult rats was significantly higher than that at the earlier stage and was the highest at the adult stage.</p><p><b>CONCLUSION</b>The expression of KiSS-1 correlates positively with that of GnRH. KiSS-1 may participate in the regulation of GnRH and is relevant to puberty onset and the regulation of reproduction function.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
ARN Mensajero
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Proteínas
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Hormona Liberadora de Gonadotropina
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Ratas Sprague-Dawley
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Kisspeptinas
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Genética
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Hipotálamo
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Metabolismo
Límite:
Animals
Idioma:
Zh
Revista:
Zhonghua nankexue
Año:
2007
Tipo del documento:
Article