Effects of a regional Chinese diet and its vitamin supplementation on proliferation of human esophageal cancer cell lines / 生物医学与环境科学（英文）
Biomedical and Environmental Sciences
; (12): 442-448, 2008.
Article
en En
| WPRIM
| ID: wpr-296026
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of a local diet popular in Yanting region (YT diet) on the proliferation of two human cell lines (Eca-109 esophageal squamous cell carcinoma line and HL7702 normal liver epithelial cell line) in rats by a sero-physiological approach.</p><p><b>METHODS</b>Male SD rats were divided into six groups and fed respectively with a conventional diet and the YT diet (one of the five experimental diets) supplemented with two vitamin mixtures (Mix. 1: vitamins A, E, and folic acid; Mix.2: mix.1 plus riboflavin and vitamin C) at two different doses. On the 30th day, sera were collected from the rats and added into a medium for cell culture, with 10% FBS used as a serum control. The effects were assessed by MTT assay, DNA synthesis and flow cytometry assays.</p><p><b>RESULTS</b>Compared with the control, the sera from rats fed with the YT diet significantly promoted the proliferation of Eca-109 cells, which was, however, reversed by the supplementation with two vitamin mixtures at high doses. Surprisingly, the same treatment produced contrary effects on HL7702 cells as compared with Eca-109 cells.</p><p><b>CONCLUSION</b>The sera from rats fed with the YT diet could promote the proliferation of human esophageal cancer cell line Eca-109, whereas the sera from those fed with the YT diet supplemented with vitamin mixtures might have inhibitory effects on the proliferation of Eca-109 cells.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Factores de Tiempo
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Vitaminas
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Neoplasias Esofágicas
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Carcinoma de Células Escamosas
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Aumento de Peso
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China
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Ratas Sprague-Dawley
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Suplementos Dietéticos
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Biología Celular
Límite:
Animals
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Humans
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Male
País/Región como asunto:
Asia
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2008
Tipo del documento:
Article